Transcapsidation involves the packing of the ITR from one particular serotype in AAV into the capsids of a different kind of serotype. The majority of cases are based on the extensively studied AAV cell line genome that is wrapped into different AAV serotypes to study the effectiveness. The disadvantage of using transpeptidation for modifying AAV tropism is the possibility of a lower titer, depending on the serotypes employed. Furthermore, due to an interaction of the C-terminus, the coat of protein on the capsid and the genome of the virus results in fewer yields from AAV particles are likely when the virus is produced.
Approaches to Gene Therapy Plasmid DNA Manufacture – Plasmid DNA is the core of viral and mRNA vector generation. It provides the coding sequences to gene-based advanced therapeutic medicinal products (ATMPs). This is why its manufacture is crucial for both supply and success of these products.
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GenScript ProBio provides one-stop stable cell line solutions including gene synthesis, vector construction, lentivirus packaging, and host cell characterization. We focus on delivering high-quality cell line free of fungus, bacteria and mycoplasma. We at GenScript ProBio have successfully constructed more than 500 assay cell lines, of which more than 100 can be used for antibody development. For more information, contact us now.