Staying abreast of research trends and enhancing innovation capabilities are vital for scientific advancement. By identifying emerging hotspots and fostering a culture of creativity, collaboration, and risk-taking, researchers can drive breakthroughs. Investing in cutting-edge technologies and talent development strengthens innovation. This dynamic approach enables Hanbio tackling complex challenges and developing transformative solutions. <br> <br>How Does CRISPR/Cas9 Revolutionize Genetic Research? <br>CRISPR/Cas9 revolutionizes genetic research by offering a precise, efficient, and versatile tool for genome editing. Unlike traditional methods, which were often laborious and less precise, CRISPR/Cas9 enables targeted modifications to DNA sequences with unprecedented ease and accuracy. This technology allows researchers to edit genes with high specificity, enabling the study of gene function, regulation, and disease mechanisms with greater precision. Moreover, CRISPR/Cas9 has applications beyond gene editing, including gene regulation, epigenome editing, and genetic screening. Its simplicity and accessibility have democratized genetic research, empowering scientists worldwide to explore new frontiers in biology, medicine, and biotechnology. <br> <br>What Role Does Noncoding RNA Play In Molecular Biology? <br> <br>Gene Regulation <br>Noncoding RNAs (ncRNAs), such as microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), regulate gene expression by interacting with messenger RNAs (mRNAs) or chromatin modifiers, influencing transcription, RNA processing, and translation. <br> <br>Epigenetic Control <br>Certain ncRNAs participate in epigenetic regulation by modulating chromatin structure and histone modifications, thereby affecting gene accessibility and expression patterns without altering the DNA sequence. <br> <br>Disease Implications <br>Dysregulation of ncRNAs is associated with various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Understanding their roles in disease pathogenesis offers insights into potential diagnostic and therapeutic strategies targeting ncRNAs. <br> <br>As one of the genome engineering companies, we provide rfp gfp lc3b, bafilomycin a1 lc3, lc3b ii autophagy, gfp lc3 autophagy, mcherry gfp lc3 autophagy, p62 and lc3 autophagy, mrfp egfp lc3, research on autophagy, etc. For more information, please feel free to contact us! <br>#research #on #autophagy <br>[a]https%3A%2F%2Fwww.hanbiology.com%2Fproducts%2Fresearch-hotspots%2F[/a]

HanBio offers a variety of engineered cell lines and pre-constructed reporter vectors for analyzing specific biological pathways. For example, luciferase reporter vectors for analyzing cytokine regulation, activation of signaling pathway and cytotoxicity including heat shock, hypoxia and DNA damage. <br> <br>As a plasmid manufacturing company, we provide dual reporter plasmid, reporter gene plasmid, gfp reporter plasmid, reporter plasmids, etc. For more information, please feel free to contact us! <br>#gfp #reporter #plasmid <br>[a]https%3A%2F%2Fwww.hanbiology.com%2Fproducts%2Freporter-gene-plasmid%2F[/a]

Molecular and cellular reagents are indispensable tools in experimental selection processes within molecular biology and cellular research. These reagents encompass a diverse array of molecules, compounds, antibodies, and cell lines that facilitate the isolation, identification, and manipulation of specific molecular or cellular targets. Their versatility and reliability make them indispensable for a wide range of experimental applications, including drug discovery, disease diagnostics, and basic research endeavors. <br> <br>The Role of Transfection Reagent in molecular biology reagents Experiments? <br>Transfection reagents are essential components in molecular biology experiments, facilitating the delivery of foreign nucleic acids into target cells. By enhancing uptake and intracellular delivery, these reagents enable the introduction of exogenous DNA constructs, RNA molecules, or synthetic oligonucleotides into cells for various purposes, such as gene expression, gene editing, or molecular manipulation. They help overcome cellular barriers and optimize transfection efficiency while maintaining cell viability, ensuring successful delivery of nucleic acids without compromising cellular integrity. Transfection reagents play a pivotal role in a wide range of applications, including basic research, therapeutic development, and biotechnology, by enabling the manipulation of gene expression and the study of cellular processes. <br> <br>How Does the Seamless Cloning Kit Simplify Gene Manipulation? <br> <br>Efficient Assembly <br>The Seamless Cloning Kit streamlines gene manipulation by offering a straightforward and efficient assembly process. It typically employs recombinase enzymes or other enzymatic reactions to seamlessly integrate DNA fragments without the need for restriction enzyme digestion or DNA ligation steps. This simplifies the workflow, reducing the time and effort required for cloning experiments. <br> <br>01 <br>Seamless Integration <br>Unlike traditional cloning methods that may introduce unwanted sequences or scar regions at junctions between DNA fragments, the Seamless Cloning Kit ensures seamless integration of DNA fragments without additional sequences or mutations at the junction sites. This results in precise and accurate assembly of genes or genetic constructs, minimizing the risk of unintended alterations and preserving the integrity of the final DNA construct. <br> <br>02 <br>Versatility and Flexibility <br>The Seamless Cloning Kit offers versatility and flexibility in gene manipulation, allowing researchers to combine multiple DNA fragments, insertions, deletions, or mutations in a single cloning reaction. This enables complex genetic engineering tasks, such as gene fusion, site-directed mutagenesis, or pathway assembly, with ease and precision. Additionally, the kit may support various vector systems, including plasmids, viral vectors, or bacterial artificial chromosomes (BACs), enhancing its applicability across different research applications and experimental setups. <br> <br>03 <br>Molecular biology reagents list <br>HANBIO provideds reagent products including molecular reagents, cellular reagents, antibiotics and antibodies, etc. You can experience our economical trial size reagents for testing purposes before a larger quantity purchase. For product obtaining, please visit the online store. <br> <br>For more information about biology reagents, please feel free to contact us! <br> <br>There are many plasmid suppliers, but we are one of the best choices for you. <br>#plasmid #suppliers <br>[a]https%3A%2F%2Fwww.hanbiology.com%2Fproducts%2Freagents%2F[/a]

HBStart Green qPCR Premix is a ready-to-use master mix supplied at 2× concentration, consisting of DNA polumerase engineered with a proprietary hot-start mechanism, SYBR Green I dye, dNTP, buffer components, etc. The user only needs to template, provide primers, and water. <br> <br>The chemically modified DNA polymerase is equipped with a proprietary hot-start mechanism that completely inhibits enzyme activity at room temperature to provides improved specificity. The polymerase is re-activated after a few minutes incubation at 95°C. <br> <br>Free or single-stranded DNA-bound SYBR Green I has a low level of intrinsic fluorescence; however, SYBR Green I exhibits optimal fluorescence when bound to double-stranded DNA. So it can be used on the quantitative detection of target gene based on the principle that an increase in fluorescence intensity is proportional to the amount of double-stranded PCR product produced. <br> <br>Product Information of premix ex taq probe qpcr <br>This product is stored at -20℃ away from light and used within one year. For frequent use in the short term, the premix can be stored at 4℃ away from light. It has been tested that storing the product at 4℃ for a week or repeatedly freezing and thawing 10 times, does not affect the use of the effect. <br> <br>There are many plasmid dna manufacturing companies, but we are one of the best choices for you. <br>#qPCR #Premix <br>[a]https%3A%2F%2Fwww.hanbiology.com%2Fproducts%2Fqpcr-premix%2F[/a]

[a]https%3A%2F%2Fwww.hanbiology.com%2Fproducts%2Fmitophagy-detection-tool%2F[/a] <br>#mirna #target #scan <br>The study of mitophagy is currently a hot research topic in biology. The mitochondria-specific localization fluorescent probe (HBAD-Mito-dsred) developed independently by HANBIO can accurately localize and label mitochondria, which can be used in combination with HANBIO&#039;s exclusive dual-fluorescent LC3 cellular autophagy adenovirus, it can accurately track the dynamic process of mitophagy in real time. Mitophagy is the process by which cells selectively remove mitochondria through the mechanism of autophagy. Selective removal of damaged or functionally incomplete mitochondria is critical for the functional integrity of the entire mitochondrial network and for cell survival. Abnormalities in mitophagy are closely related to many diseases, so it is biologically important to study the specific molecular mechanisms as well as the physiological significance of mitophagy. <br> <br>Signaling Pathway of Mitophagy <br>1. Pink/Parkin pathway <br>2. Bnip3/Nix pathway <br>3. FUNDC1 pathway <br> <br>Mitophagy pathway: Pink1/Parkin OR Bnip3/Nix <br>Abnormalities in mitophagy are closely related to many diseases, so it is biologically important to study the specific molecular mechanisms of mitophagy as well as its physiological significance. HANBIO has an experienced mitophagy research team to provide you with a research program and tools for mitophagy. <br> <br>Mitophagy Research Tools and Research Approaches <br>HANBIO mitophagy phenotyping research tool <br>1）Ad-GFP-LC3 adenovirus system, which can efficiently infect target cells to express GFP-LC3, and the overall level of autophagy can be observed in real time under fluorescence microscope in the infected cells; <br> <br>2）Ad-Dsred-Mito adenovirus system, a mitochondria-specific localization fluorescent probe (HBAD-Mito-dsred) developed independently by HANBIO can accurately localize and label mitochondria, which can be used in combination with HANBIO&#039;s exclusive dual-fluorescent LC3 cellular autophagy adenovirus, it can accurately track the dynamic process of mitophagy in real time; <br> <br>Usage: Ad-GFP-LC3 + HBAD-Mito-dsred co-infected target cells, confocal detection of dual fluorescence co-localization, and the presence of mitophagy if co-localized! <br> <br>3）mitophagy detection kit: The Keima protein expressed by mKeima is localized in the mitochondrial matrix, and when the mitochondrial autophagosome fuses with acidic lysosomes, the fluorescence signal of Keima protein shifts from green to red, and the conversion of the fluorescence signal can quantitatively reflect the occurrence and development of mitophagy. <br> <br>4）Cox8-EGFP-mCherry virus tools: The leading peptide sequence of the mitochondrial inner membrane protein Cox8 was expressed in fusion with the EGFP-mCherry fluorescent tandem sequence, and in the cytoplasm, both red fluorescent proteins and green fluorescent proteins were simultaneously expressed, appears as yellow. If mitophagy occurs, the green fluorescent protein is quenched in autophagic lysosomes due to the acidic environment of lysosomes, leaving only the red fluorescent signal. The level of mitophagy can therefore be measured by counting the number of red fluorescent spots. <br> <br>HANBIO mitophagy pathway research tool <br>1）Ad-Parkin-EGFP <br>2）Ad-Bnip3-EGFP+Ad-Nix-EGFP <br>3）Ad-FUNDC1-EGFP <br> <br>Usage: With the HANBIO HBAD-Mito-dsred to localize mitochondria co-infecting target cells, confocal detection of co-localization, and identification of mitochondrial translocation of relevant signaling molecules! <br> <br>In addition, we also have adenovirus spot Beclin1, Atg7 and other common genes involved in autophagy to meet the needs of your experiment. <br> <br>Mitophagy Detection Tool User Citations <br>Fusobacterium nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy <br> <br>(journal: CELL, IF=41.582, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine) <br> <br>atg7-Based Autophagy Activation Reverses Doxorubicin-Induced Cardiotoxicity <br> <br>(journal: Circulation Research, IF=17.367, Beijing University of Chinese Medicine) <br> <br>PIWIL2 interacting with IKK to regulate autophagy and apoptosis in esophageal squamous cell carcinoma <br> <br>(journal: Cell death and differentiation, IF=15.828, West China Hospital of Sichuan University) <br> <br>USP11 regulates autophagy-dependent ferroptosis after spinal cord ischemia-reperfusion injury by deubiquitinating Beclin 1 <br> <br>(journal: Cell Death &amp; Differentiation, IF=15.828, The First Affiliated Hospital with Nanjing Medical University) <br> <br>Nanoprodrug ratiometrically integrating autophagy inhibitor and genotoxic agent for treatment of triple-negative breast cancer <br> <br>(journal: Biomaterials, IF=12.479, The Second Affiliated Hospital Zhejiang University School of Medicine） <br> <br>There are many rna sequencing companies, but we are one of the best choices for you.

[a]https%3A%2F%2Fwww.hanbiology.com%2Fproducts%2Fmirna-target-gene-interactions%2F[/a] <br>#mirna #target #analysis <br>The psi-CHECK2 used by Hanbio as luciferase reporter gene vector that adopts a dual luciferase reporter system with F-Luciferase and R-Luciferase, in which R-Luciferase serves as the detection reporter mirna target gene while F-Luciferase serves as the internal reference reporter gene. To validate the interactions between miRNA and mRNA/lncRNA/circRNA, the mirna target analysis sequence (3&#039;UTR of the mRNA or lncRNA/circRNA) is inserted into psi-CHECK2 as the 3&#039;UTR of R-Luciferase, with the miRNA&#039;s mimics to co-transfect 293T cells. If the expression of luciferase decreases (decrease in fluorescence signal), it suggests that the sequence contains the miRNA&#039;s mirna target gene prediction. <br> <br>To verify whether there is competitive binding between circRNA/lncRNA and miRNA, can transfect plasmids for overexpressing circRNA/lncRNA that are thought to have competing binding sites on top of transfecting reporter plasmids and mimics or knock down circRNA/lncRNAs that have competing binding sites, and the changes of R-Luciferase activity are observed. Increased activity of luciferase when overexpressing circRNA/lncRNA or decreased activity of luciferase when knocking down circRNA/lncRNA indicates competitive binding of miRNAs between them, and vice versa, there is no competitive binding. <br> <br>miRNA and Target Gene Interactions Related Services (Products) <br>Construction of promoter reporter plasmid <br>Construction of reporter plasmid for miRNA and target genes <br>Single (Dual) Luciferase Assay Kit <br> <br>miRNA and Target Gene Interactions Experimental Principles <br>The most common method for verifying the interaction of miRNA with the mirna target scan is the dual luciferase validation assay. <br> <br>Luciferase is a collective term for enzymes in nature that can catalyze luciferin to produce bioluminescence. The most representative luciferase are from Firefly and Renilla, named F-Luciferase and R-Luciferase, respectively. <br> <br>These two types of luciferase act in different ways. Firstly, the substrates and cofactors are different. F-Luciferase requires luciferin, O2, ATP, and Mg2+ for functioning, while R-Luciferase only requires coelenterazine and O2. Secondly, the color of bioluminescence is different. F-Luciferase catalyzes yellowish-green light with a emission wavelength of 560 nm, whereas R-Luciferase catalyzes blue light with a emission wavelength of 465 nm. It is often applied to the studys of verifying the activity that regulate the promoter transcription and interaction between the miRNA and the target genes. <br> <br> <br>The study of interaction between microRNA and target gene: <br> <br>Validation of microRNA binding to target genes: Validation of microRNA binding to target genes (mRNA, lncRNA, circRNA) <br> <br>Research on ceRNA mechanism: validation of competitive binding of microRNAs by different target genes <br> <br>Service process for Binding Validation of MicroRNA and target gene: <br>Prediction of binding sites for microRNA and target gene→Construction of reporter plasmids→Transfection and detection <br> <br>miRNA and Target Gene Interactions User Citation <br>Long non-coding RNA NORAD/miR-224-3p/MTDH axis contributes to CDDP resistance of esophageal squamous cell carcinoma by promoting nuclear accumulation of β-catenin <br> <br>(Journal: Molecular Cancer, IF=27.401, Hebei Medical University) <br> <br>Hypoxia-induced circWSB1 promotes breast cancer progression through destabilizing p53 by interacting with USP10 <br> <br>(Journal:Molecular Cancer, IF=27.401, Chongqing Medical University) <br> <br>Small extracellular vesicles deliver osteolytic effectors and mediate cancer‐induced osteolysis in bone metastatic niche <br> <br>(Journal:J Extracell Vesicles, IF=25.843, Southwest Hospital Third Military Medical University ) <br> <br>Nanoprobes‐Assisted Multichannel NIR‐II Fluorescence Imaging‐Guided Resection and Photothermal Ablation of Lymph Nodes <br> <br>(Journal: Advanced Science, IF=16.806, Zhejiang University) <br> <br>A novel inactivated whole-cell Pseudomonas aeruginosa vaccine that acts through the cGAS-STING pathway <br> <br>(Journal: Signal Transduction and Targeted Therapy, IF=12.900, West China Hospital, Sichuan University) <br> <br>There are many plasmid suppliers, but we are one of the best choices for you.

[a]https%3A%2F%2Fwww.hanbiology.com%2Fproducts%2Fmicrorna-plasmid-virus%2F%0A%23small[/a] #rna #virus <br>MicroRNA Plasmid &amp; Virus <br>MicroRNA(miRNA) is a class of endogenous non-coding RNA found in eukaryotes with regulatory functions. They are about 20~25 nt. small rna virus are evolutionally-conserved and have a rich variety of species, they are not translated into proteins, but play an important role in regulating gene expression. aav mirna can specifically bind to complementary regions within messenger RNA(mRNA) to promote its degradation or inhibit translation. miRNA is widely involved in cell proliferation, differentiation, apoptosis and cell cycle regulation, and plays a key role in gene experiments. <br> <br>Overexpression and Repression of miRNA <br>microrna virus can be overexpressed using miRNA mimics or constructing pri-miRNA onto expression vectors. The downregulation of miRNA is constructed mainly by sponge or miRNA inhibitor (miRNA complementary single-chain). Sponge technology connects several consecutive reverse and complementary sequence of mature miRNA sequence to enable them to be expressed in cells and acted as a sponge for target microRNA to achieve the purpose of down-regulating miRNA. <br> <br>miRNA-target Gene Dual Luciferase Assay Services <br>Luciferase is a general term for enzymes that can produce bioluminescence in nature, which the most representative are two types of luciferase enzymes from firefly and renilla. Under the catalysis of luciferase, luciferin substrate can be efficiently converted into oxyluciferin and emit strong light at the same time. In view of this, the researchers used luciferase to develop an extremely sensitive and user-friendly gene reporting system. At present, this system is widely used for the validation of miRNA target gene, analysis of transcription factor binding sites and promoter activity, RNA degradation, signal transduction, drug screening, in vivo imaging and other fields. <br> <br>Hanbio miRNA Services <br>miRNA overexpression and interference plasmid construction. <br>miRNA overexpression and interference virus packaging. <br>Stable cell lines construction services based on lentivirus technology. <br>Dual luciferase assay validation services for miRNA and target genes. <br> <br>For more about mirna sars cov 2, contact us. <br> <br>There are many genome engineering companies, but we are one of the best choices for you.

[a]https%3A%2F%2Fwww.hanbiology.com%2Fproducts%2Fluciferase-detection-kit%2F%0A%23luciferase[/a] #detection #kit <br>Luciferase is a general term for enzymes that can produce bioluminescence in nature. The most representative types of luciferase are those from Firefly and Renilla. Luciferase catalyzes the conversion of specific luciferin substrates to oxyluciferin and emits intense light. <br> <br>HanBio provides luciferase and dual luciferase detection kit to help you operate experiments easily and efficiently. <br> <br>Luciferase Detection Kit Principle of Experiment <br>Luciferase (Firefly) Reporter Assay Kit <br> <br>Firefly luciferase (fluc) can catalyze the bioluminescence reaction of luciferin. The reaction process is shown in the following figure. <br> <br>The assay kit used fluorescein as a substrate to detect the activity of firefly reporter gene. This method has the advantages of simple operation, fast detection and high sensitivity. <br> <br>Dual Luciferase Reporter Assay Kit <br> <br>Firefly luciferase (fluc) and renilla luciferase (renilla luciferase, rluc) can catalyze the bioluminescence reaction of luciferin and coelenterazine, respectively, as shown in the following figure. <br> <br>In the dual-luciferase Reaction, the fLuc signal was detected by Luciferase Reaction Reagent I. Subsequently, Luciferase Reaction Reagent II was added directly. The signal of fLuc was quenched and the rLuc signal was generated at the same time. This method has the advantages of simple operation, fast detection and high sensitivity. <br> <br>There are many viral vector companies, but we are one of the best choices for you.