HBStart Green qPCR Premix is a ready-to-use master mix supplied at 2× concentration, consisting of DNA polumerase engineered with a proprietary hot-start mechanism, SYBR Green I dye, dNTP, buffer components, etc. The user only needs to template, provide primers, and water. <br> <br>The chemically modified DNA polymerase is equipped with a proprietary hot-start mechanism that completely inhibits enzyme activity at room temperature to provides improved specificity. The polymerase is re-activated after a few minutes incubation at 95°C. <br> <br>Free or single-stranded DNA-bound SYBR Green I has a low level of intrinsic fluorescence; however, SYBR Green I exhibits optimal fluorescence when bound to double-stranded DNA. So it can be used on the quantitative detection of target gene based on the principle that an increase in fluorescence intensity is proportional to the amount of double-stranded PCR product produced. <br> <br>Product Information of premix ex taq probe qpcr <br>This product is stored at -20℃ away from light and used within one year. For frequent use in the short term, the premix can be stored at 4℃ away from light. It has been tested that storing the product at 4℃ for a week or repeatedly freezing and thawing 10 times, does not affect the use of the effect. <br> <br>There are many plasmid dna manufacturing companies, but we are one of the best choices for you. <br>#qPCR #Premix <br>[a]https%3A%2F%2Fwww.hanbiology.com%2Fproducts%2Fqpcr-premix%2F[/a]

[a]https%3A%2F%2Fwww.hanbiology.com%2Fproducts%2Fmirna-target-gene-interactions%2F[/a] <br>#mirna #target #analysis <br>The psi-CHECK2 used by Hanbio as luciferase reporter gene vector that adopts a dual luciferase reporter system with F-Luciferase and R-Luciferase, in which R-Luciferase serves as the detection reporter mirna target gene while F-Luciferase serves as the internal reference reporter gene. To validate the interactions between miRNA and mRNA/lncRNA/circRNA, the mirna target analysis sequence (3&#039;UTR of the mRNA or lncRNA/circRNA) is inserted into psi-CHECK2 as the 3&#039;UTR of R-Luciferase, with the miRNA&#039;s mimics to co-transfect 293T cells. If the expression of luciferase decreases (decrease in fluorescence signal), it suggests that the sequence contains the miRNA&#039;s mirna target gene prediction. <br> <br>To verify whether there is competitive binding between circRNA/lncRNA and miRNA, can transfect plasmids for overexpressing circRNA/lncRNA that are thought to have competing binding sites on top of transfecting reporter plasmids and mimics or knock down circRNA/lncRNAs that have competing binding sites, and the changes of R-Luciferase activity are observed. Increased activity of luciferase when overexpressing circRNA/lncRNA or decreased activity of luciferase when knocking down circRNA/lncRNA indicates competitive binding of miRNAs between them, and vice versa, there is no competitive binding. <br> <br>miRNA and Target Gene Interactions Related Services (Products) <br>Construction of promoter reporter plasmid <br>Construction of reporter plasmid for miRNA and target genes <br>Single (Dual) Luciferase Assay Kit <br> <br>miRNA and Target Gene Interactions Experimental Principles <br>The most common method for verifying the interaction of miRNA with the mirna target scan is the dual luciferase validation assay. <br> <br>Luciferase is a collective term for enzymes in nature that can catalyze luciferin to produce bioluminescence. The most representative luciferase are from Firefly and Renilla, named F-Luciferase and R-Luciferase, respectively. <br> <br>These two types of luciferase act in different ways. Firstly, the substrates and cofactors are different. F-Luciferase requires luciferin, O2, ATP, and Mg2+ for functioning, while R-Luciferase only requires coelenterazine and O2. Secondly, the color of bioluminescence is different. F-Luciferase catalyzes yellowish-green light with a emission wavelength of 560 nm, whereas R-Luciferase catalyzes blue light with a emission wavelength of 465 nm. It is often applied to the studys of verifying the activity that regulate the promoter transcription and interaction between the miRNA and the target genes. <br> <br> <br>The study of interaction between microRNA and target gene: <br> <br>Validation of microRNA binding to target genes: Validation of microRNA binding to target genes (mRNA, lncRNA, circRNA) <br> <br>Research on ceRNA mechanism: validation of competitive binding of microRNAs by different target genes <br> <br>Service process for Binding Validation of MicroRNA and target gene: <br>Prediction of binding sites for microRNA and target gene→Construction of reporter plasmids→Transfection and detection <br> <br>miRNA and Target Gene Interactions User Citation <br>Long non-coding RNA NORAD/miR-224-3p/MTDH axis contributes to CDDP resistance of esophageal squamous cell carcinoma by promoting nuclear accumulation of β-catenin <br> <br>(Journal: Molecular Cancer, IF=27.401, Hebei Medical University) <br> <br>Hypoxia-induced circWSB1 promotes breast cancer progression through destabilizing p53 by interacting with USP10 <br> <br>(Journal:Molecular Cancer, IF=27.401, Chongqing Medical University) <br> <br>Small extracellular vesicles deliver osteolytic effectors and mediate cancer‐induced osteolysis in bone metastatic niche <br> <br>(Journal:J Extracell Vesicles, IF=25.843, Southwest Hospital Third Military Medical University ) <br> <br>Nanoprobes‐Assisted Multichannel NIR‐II Fluorescence Imaging‐Guided Resection and Photothermal Ablation of Lymph Nodes <br> <br>(Journal: Advanced Science, IF=16.806, Zhejiang University) <br> <br>A novel inactivated whole-cell Pseudomonas aeruginosa vaccine that acts through the cGAS-STING pathway <br> <br>(Journal: Signal Transduction and Targeted Therapy, IF=12.900, West China Hospital, Sichuan University) <br> <br>There are many plasmid suppliers, but we are one of the best choices for you.

[a]https%3A%2F%2Fwww.hanbiology.com%2Fproducts%2Fluciferase-detection-kit%2F%0A%23luciferase[/a] #detection #kit <br>Luciferase is a general term for enzymes that can produce bioluminescence in nature. The most representative types of luciferase are those from Firefly and Renilla. Luciferase catalyzes the conversion of specific luciferin substrates to oxyluciferin and emits intense light. <br> <br>HanBio provides luciferase and dual luciferase detection kit to help you operate experiments easily and efficiently. <br> <br>Luciferase Detection Kit Principle of Experiment <br>Luciferase (Firefly) Reporter Assay Kit <br> <br>Firefly luciferase (fluc) can catalyze the bioluminescence reaction of luciferin. The reaction process is shown in the following figure. <br> <br>The assay kit used fluorescein as a substrate to detect the activity of firefly reporter gene. This method has the advantages of simple operation, fast detection and high sensitivity. <br> <br>Dual Luciferase Reporter Assay Kit <br> <br>Firefly luciferase (fluc) and renilla luciferase (renilla luciferase, rluc) can catalyze the bioluminescence reaction of luciferin and coelenterazine, respectively, as shown in the following figure. <br> <br>In the dual-luciferase Reaction, the fLuc signal was detected by Luciferase Reaction Reagent I. Subsequently, Luciferase Reaction Reagent II was added directly. The signal of fLuc was quenched and the rLuc signal was generated at the same time. This method has the advantages of simple operation, fast detection and high sensitivity. <br> <br>There are many viral vector companies, but we are one of the best choices for you.